Ontology highlight
ABSTRACT:
SUBMITTER: Gupta S
PROVIDER: S-EPMC3443160 | biostudies-literature | 2012 Sep
REPOSITORIES: biostudies-literature
Gupta Sayan S D'Mello Rhijuta R Chance Mark R MR
Proceedings of the National Academy of Sciences of the United States of America 20120827 37
Water is critical for the structure, stability, and functions of macromolecules. Diffraction and NMR studies have revealed structure and dynamics of bound waters at atomic resolution. However, localizing the sites and measuring the dynamics of bound waters, particularly on timescales relevant to catalysis and macromolecular assembly, is quite challenging. Here we demonstrate two techniques: first, temperature-dependent radiolytic hydroxyl radical labeling with a mass spectrometry (MS)-based read ...[more]