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Protein Tertiary Structure by Crosslinking/Mass Spectrometry.

ABSTRACT: Observing the structures of proteins within the cell and tracking structural changes under different cellular conditions are the ultimate challenges for structural biology. This, however, requires an experimental technique that can generate sufficient data for structure determination and is applicable in the native environment of proteins. Crosslinking/mass spectrometry (CLMS) and protein structure determination have recently advanced to meet these requirements and crosslinking-driven de novo structure determination in native environments is now possible. In this opinion article, we highlight recent successes in the field of CLMS with protein structure modeling and challenges it still holds.

SUBMITTER: Schneider M

PROVIDER: S-EPMC5854373 | biostudies-other | 2018 Mar

REPOSITORIES: biostudies-other

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Protein Tertiary Structure by Crosslinking/Mass Spectrometry.

Schneider Michael M Belsom Adam A Rappsilber Juri J

Trends in biochemical sciences 20180131 3

Observing the structures of proteins within the cell and tracking structural changes under different cellular conditions are the ultimate challenges for structural biology. This, however, requires an experimental technique that can generate sufficient data for structure determination and is applicable in the native environment of proteins. Crosslinking/mass spectrometry (CLMS) and protein structure determination have recently advanced to meet these requirements and crosslinking-driven de novo st ...[more]

PMID: 29395654

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