Metagenomic microbial community profiling using unique clade-specific marker genes.
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ABSTRACT:
SUBMITTER: Segata N
PROVIDER: S-EPMC3443552 | biostudies-literature | 2012 Jun
REPOSITORIES: biostudies-literature
Publications
Metagenomic microbial community profiling using unique clade-specific marker genes.
Segata Nicola N Waldron Levi L Ballarini Annalisa A Narasimhan Vagheesh V Jousson Olivier O Huttenhower Curtis C
Nature methods 20120610 8
Metagenomic shotgun sequencing data can identify microbes populating a microbial community and their proportions, but existing taxonomic profiling methods are inefficient for increasingly large data sets. We present an approach that uses clade-specific marker genes to unambiguously assign reads to microbial clades more accurately and >50× faster than current approaches. We validated our metagenomic phylogenetic analysis tool, MetaPhlAn, on terabases of short reads and provide the largest metagen ...[more]
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