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Development of a serum-based Taqman real-time PCR assay for diagnosis of invasive aspergillosis.

ABSTRACT: We compared an Aspergillus fumigatus-specific Taqman real-time PCR assay for the diagnosis of invasive aspergillosis (IA) with the enzyme-linked immunosorbent assay Platelia Aspergillus method. Patients with proven or probable IA had positive results with at least one of the two methods. The PCR method seems to be more specific, and a combination of the two should improve the diagnosis of IA.

SUBMITTER: Challier S

PROVIDER: S-EPMC344496 | biostudies-literature | 2004 Feb

REPOSITORIES: biostudies-literature

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Development of a serum-based Taqman real-time PCR assay for diagnosis of invasive aspergillosis.

Challier Svetlana S Boyer Stéphanie S Abachin Eric E Berche Patrick P

Journal of clinical microbiology 20040201 2

We compared an Aspergillus fumigatus-specific Taqman real-time PCR assay for the diagnosis of invasive aspergillosis (IA) with the enzyme-linked immunosorbent assay Platelia Aspergillus method. Patients with proven or probable IA had positive results with at least one of the two methods. The PCR method seems to be more specific, and a combination of the two should improve the diagnosis of IA. ...[more]

PMID: 14766869

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