Ontology highlight
ABSTRACT:
SUBMITTER: Shi X
PROVIDER: S-EPMC3445270 | biostudies-literature | 2012 Apr
REPOSITORIES: biostudies-literature
Shi Xinghua X Jung Yonil Y Lin Li-Jung LJ Liu Cheng C Wu Cong C Cann Isaac K O IK Ha Taekjip T
Nature methods 20120401 5
A major hurdle for molecular mechanistic studies of many proteins is the lack of a general method for fluorescence labeling with high efficiency, specificity and speed. By incorporating an aldehyde motif genetically into a protein and improving the labeling kinetics substantially under mild conditions, we achieved fast, site-specific labeling of a protein with ∼100% efficiency while maintaining the biological function. We show that an aldehyde-tagged protein can be specifically labeled in cell e ...[more]