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Graftable SCoMPIs enable the labeling and X-ray fluorescence imaging of proteins.

ABSTRACT: Bio-imaging techniques alternative to fluorescence microscopy are gaining increasing interest as complementary tools to visualize and analyze biological systems. Among them, X-ray fluorescence microspectroscopy provides information on the local content and distribution of heavy elements (Z ? 14) in cells or biological samples. In this context, similar tools to those developed for fluorescence microscopy are desired, including chemical probes or tags. In this work, we study rhenium complexes as a convenient and sensitive probe for X-ray fluorescence microspectroscopy. We demonstrate their ability to label and sense exogenously incubated or endogenous proteins inside cells.

SUBMITTER: Hostachy S

PROVIDER: S-EPMC5958345 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Graftable SCoMPIs enable the labeling and X-ray fluorescence imaging of proteins.

Hostachy Sarah S Masuda Marie M Miki Takayuki T Hamachi Itaru I Sagan Sandrine S Lequin Olivier O Medjoubi Kadda K Somogyi Andrea A Delsuc Nicolas N Policar Clotilde C

Chemical science 20180413 19

Bio-imaging techniques alternative to fluorescence microscopy are gaining increasing interest as complementary tools to visualize and analyze biological systems. Among them, X-ray fluorescence microspectroscopy provides information on the local content and distribution of heavy elements (<i>Z</i> ≥ 14) in cells or biological samples. In this context, similar tools to those developed for fluorescence microscopy are desired, including chemical probes or tags. In this work, we study rhenium complex ...[more]

PMID: 29896390

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