A high-throughput approach to identify genomic variants of bacterial metabolite producers at the single-cell level.
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ABSTRACT:
SUBMITTER: Binder S
PROVIDER: S-EPMC3446293 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
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A high-throughput approach to identify genomic variants of bacterial metabolite producers at the single-cell level.
Binder Stephan S Schendzielorz Georg G Stäbler Norma N Krumbach Karin K Hoffmann Kristina K Bott Michael M Eggeling Lothar L
Genome biology 20120528 5
We present a novel method for visualizing intracellular metabolite concentrations within single cells of Escherichia coli and Corynebacterium glutamicum that expedites the screening process of producers. It is based on transcription factors and we used it to isolate new L-lysine producing mutants of C. glutamicum from a large library of mutagenized cells using fluorescence-activated cell sorting (FACS). This high-throughput method fills the gap between existing high-throughput methods for mutant ...[more]
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