Unknown

Dataset Information

0

Interplay between cytoskeletal stresses and cell adaptation under chronic flow.


ABSTRACT: Using stress sensitive FRET sensors we have measured cytoskeletal stresses in α-actinin and the associated reorganization of the actin cytoskeleton in cells subjected to chronic shear stress. We show that long-term shear stress reduces the average actinin stress and this effect is reversible with removal of flow. The flow-induced changes in cytoskeletal stresses are found to be dynamic, involving a transient decrease in stress (phase-I), a short-term increase (3-6 min) (Phase-II), followed by a longer-term decrease that reaches a minimum in ~20 min (Phase-III), before saturating. These changes are accompanied by reorganization of the actin cytoskeleton from parallel F-actin bundles to peripheral bundles. Blocking mechanosensitive ion channels (MSCs) with Gd(3+) and GsMTx4 (a specific inhibitor) eliminated the changes in cytoskeletal stress and the corresponding actin reorganization, indicating that Ca(2+) permeable MSCs participate in the signaling cascades. This study shows that shear stress induced cell adaptation is mediated via MSCs.

SUBMITTER: Verma D 

PROVIDER: S-EPMC3446919 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC4955960 | biostudies-literature
| S-EPMC2965954 | biostudies-literature
| S-EPMC3107263 | biostudies-other
| S-EPMC4893201 | biostudies-literature
| S-EPMC9458118 | biostudies-literature
| S-EPMC10851303 | biostudies-literature
| S-EPMC3164210 | biostudies-literature
| S-EPMC3813296 | biostudies-literature
| S-EPMC8259999 | biostudies-literature
| S-EPMC3216658 | biostudies-other