Ontology highlight
ABSTRACT:
SUBMITTER: Burgess A
PROVIDER: S-EPMC3447815 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Burgess Andrew A Lorca Thierry T Castro Anna A
PloS one 20120920 9
Historically, the analysis of DNA replication in mammalian tissue culture cells has been limited to static time points, and the use of nucleoside analogues to pulse-label replicating DNA. Here we characterize for the first time a novel Chromobody cell line that specifically labels endogenous PCNA. By combining this with high-resolution confocal time-lapse microscopy, and with a simplified analysis workflow, we were able to produce highly detailed, reproducible, quantitative 4D data on endogenous ...[more]