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A facile method to synthesize histones with posttranslational modification mimics.

ABSTRACT: Using an evolved pyrrolysyl-tRNA synthetase-tRNA(Pyl) pair, a Se-alkylselenocysteine was genetically incorporated into histone H3 with a high protein expression yield. Quantitative oxidative elimination of Se-alkylselenocysteine followed by Michael addition reactions with various thiol nucleophiles generated biologically active mimics of H3 with posttranslational modifications including lysine methylation, lysine acetylation, and serine phosphorylation.

SUBMITTER: Wang ZU

PROVIDER: S-EPMC3448024 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature

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A facile method to synthesize histones with posttranslational modification mimics.

Wang Zhiyong U ZU Wang Yane-Shih YS Pai Pei-Jing PJ Russell William K WK Russell David H DH Liu Wenshe R WR

Biochemistry 20120619 26

Using an evolved pyrrolysyl-tRNA synthetase-tRNA(Pyl) pair, a Se-alkylselenocysteine was genetically incorporated into histone H3 with a high protein expression yield. Quantitative oxidative elimination of Se-alkylselenocysteine followed by Michael addition reactions with various thiol nucleophiles generated biologically active mimics of H3 with posttranslational modifications including lysine methylation, lysine acetylation, and serine phosphorylation. ...[more]

PMID: 22697363

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