Ontology highlight
ABSTRACT:
SUBMITTER: Tavares RL
PROVIDER: S-EPMC3454941 | biostudies-literature | 2007 Aug
REPOSITORIES: biostudies-literature
Tavares Rubens L C RL Xu Kangpu K Zhang Chenyang C de Freitas Vilmon V
Journal of assisted reproduction and genetics 20070717 8
<h4>Purpose</h4>To study gene expression at single embryonic stem cell colony levels with a new RT-PCR protocol.<h4>Methods</h4>Forty-five mouse ES cell colonies were retrieved at the 5th, 10th, 15th, 20th and 25th passages. The pluripotent state was analyzed for OCT-4 and Nanog, and beta-actin as a control for the presence of templates. RT-PCR was done using the SuperScript III CellsDirect cDNA Synthesis System. Every 2 or 3 days just before passage, a single colony was loaded into a 0.5 ml PCR ...[more]