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Coilin levels modulate cell cycle progression and ?H2AX levels in etoposide treated U2OS cells.

ABSTRACT: Coilin is considered the Cajal body (CB) marker protein. In this report, we investigated the role of coilin in the DNA damage response and found that coilin reduction correlated with significantly increased levels of soluble ?H2AX in etoposide treated U2OS cells. Additionally, coilin levels influenced the proliferation rate and cell cycle distribution of cells exposed to etoposide. Moreover, coilin overexpression inhibited nucleolar localization of endogenous coilin in etoposide treated U2OS cells. Collectively, these data provide additional evidence for coilin and CBs in the DNA damage response.

SUBMITTER: Velma V

PROVIDER: S-EPMC3455142 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature

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Coilin levels modulate cell cycle progression and γH2AX levels in etoposide treated U2OS cells.

Velma Venkatramreddy V Carrero Zunamys I ZI Allen Carmen B CB Hebert Michael D MD

FEBS letters 20120807 19

Coilin is considered the Cajal body (CB) marker protein. In this report, we investigated the role of coilin in the DNA damage response and found that coilin reduction correlated with significantly increased levels of soluble γH2AX in etoposide treated U2OS cells. Additionally, coilin levels influenced the proliferation rate and cell cycle distribution of cells exposed to etoposide. Moreover, coilin overexpression inhibited nucleolar localization of endogenous coilin in etoposide treated U2OS cel ...[more]

PMID: 22986342

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