Microfluidic PCR combined with pyrosequencing for identification of allelic variants with phenotypic associations among targeted Salmonella genes.
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ABSTRACT:
SUBMITTER: Yue M
PROVIDER: S-EPMC3457111 | biostudies-literature | 2012 Oct
REPOSITORIES: biostudies-literature
Publications
Microfluidic PCR combined with pyrosequencing for identification of allelic variants with phenotypic associations among targeted Salmonella genes.
Yue Min M Schmieder Robert R Edwards Robert A RA Rankin Shelley C SC Schifferli Dieter M DM
Applied and environmental microbiology 20120810 20
A novel targeted massive parallel sequencing approach identified genetic variation in eight known or predicted fimbrial adhesins for 46 Salmonella strains. The results highlight associations between specific adhesin alleles, host species, and antimicrobial resistance. The differentiation of allelic variants has potential applications for diagnostic microbiology and epidemiological investigations. ...[more]
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