Quantifying RNA allelic ratios by microfluidic multiplex PCR and sequencing.
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ABSTRACT:
SUBMITTER: Zhang R
PROVIDER: S-EPMC3877737 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
Publications
Quantifying RNA allelic ratios by microfluidic multiplex PCR and sequencing.
Zhang Rui R Li Xin X Ramaswami Gokul G Smith Kevin S KS Turecki Gustavo G Montgomery Stephen B SB Li Jin Billy JB
Nature methods 20131124 1
We developed a targeted RNA sequencing method that couples microfluidics-based multiplex PCR and deep sequencing (mmPCR-seq) to uniformly and simultaneously amplify up to 960 loci in 48 samples independently of their gene expression levels and to accurately and cost-effectively measure allelic ratios even for low-quantity or low-quality RNA samples. We applied mmPCR-seq to RNA editing and allele-specific expression studies. mmPCR-seq complements RNA-seq for studying allelic variations in the tra ...[more]
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