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Regulatable vectors for environmental gene expression in Alphaproteobacteria.

ABSTRACT: Two expression vectors utilizing the inducible taurine promoter (tauAp) were developed. Plasmid pLMB51 is a stable low-copy vector enabling expression in the environment and in planta. The higher copy number pLMB509 enables BD restriction-independent cloning, expression, and purification of polyhistidine-tagged proteins.

SUBMITTER: Tett AJ 

PROVIDER: S-EPMC3457481 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature

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Regulatable vectors for environmental gene expression in Alphaproteobacteria.

Tett Adrian J AJ   Rudder Steven J SJ   Bourdès Alexandre A   Karunakaran Ramakrishnan R   Poole Philip S PS  

Applied and environmental microbiology 20120720 19

Two expression vectors utilizing the inducible taurine promoter (tauAp) were developed. Plasmid pLMB51 is a stable low-copy vector enabling expression in the environment and in planta. The higher copy number pLMB509 enables BD restriction-independent cloning, expression, and purification of polyhistidine-tagged proteins. ...[more]

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