Regulatable vectors for environmental gene expression in Alphaproteobacteria.
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ABSTRACT:
SUBMITTER: Tett AJ
PROVIDER: S-EPMC3457481 | biostudies-literature | 2012 Oct
REPOSITORIES: biostudies-literature
Publications
Regulatable vectors for environmental gene expression in Alphaproteobacteria.
Tett Adrian J AJ Rudder Steven J SJ Bourdès Alexandre A Karunakaran Ramakrishnan R Poole Philip S PS
Applied and environmental microbiology 20120720 19
Two expression vectors utilizing the inducible taurine promoter (tauAp) were developed. Plasmid pLMB51 is a stable low-copy vector enabling expression in the environment and in planta. The higher copy number pLMB509 enables BD restriction-independent cloning, expression, and purification of polyhistidine-tagged proteins. ...[more]
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