Ontology highlight
ABSTRACT:
SUBMITTER: Aubry A
PROVIDER: S-EPMC3457548 | biostudies-literature | 2012 Oct
REPOSITORIES: biostudies-literature
Aubry Annie A Hussack Greg G Chen Wangxue W KuoLee Rhonda R Twine Susan M SM Fulton Kelly M KM Foote Simon S Carrillo Catherine D CD Tanha Jamshid J Logan Susan M SM
Infection and immunity 20120730 10
We show in this study that toxin production in Clostridium difficile is altered in cells which can no longer form flagellar filaments. The impact of inactivation of fliC, CD0240, fliF, fliG, fliM, and flhB-fliR flagellar genes upon toxin levels in culture supernatants was assessed using cell-based cytotoxicity assay, proteomics, immunoassay, and immunoblotting approaches. Each of these showed that toxin levels in supernatants were significantly increased in a fliC mutant compared to that in the ...[more]