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Autoproteolytic fragments are intermediates in the oligomerization/aggregation of the Parkinson's disease protein alpha-synuclein as revealed by ion mobility mass spectrometry.

ABSTRACT: Gas-phase protein separation by ion mobility: With its ability to separate the Parkinson's disease protein ?-synuclein and its autoproteolytic products-despite the small concentrations of the latter-ion-mobility MS has enabled the characterization of intermediate fragments in in vitro oligomerization-aggregation. In particular, a possible key fragment, the highly aggregating C-terminal fragment, ?Syn(72-140), has been revealed.

SUBMITTER: Vlad C

PROVIDER: S-EPMC3461308 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature

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Autoproteolytic fragments are intermediates in the oligomerization/aggregation of the Parkinson's disease protein alpha-synuclein as revealed by ion mobility mass spectrometry.

Vlad Camelia C Lindner Kathrin K Karreman Christiaan C Schildknecht Stefan S Leist Marcel M Tomczyk Nick N Rontree John J Langridge James J Danzer Karin K Ciossek Thomas T Petre Alina A Gross Michael L ML Hengerer Bastian B Przybylski Michael M

Chembiochem : a European journal of chemical biology 20111107 18

Gas-phase protein separation by ion mobility: With its ability to separate the Parkinson's disease protein α-synuclein and its autoproteolytic products-despite the small concentrations of the latter-ion-mobility MS has enabled the characterization of intermediate fragments in in vitro oligomerization-aggregation. In particular, a possible key fragment, the highly aggregating C-terminal fragment, αSyn(72-140), has been revealed. ...[more]

PMID: 22162214

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