Unknown

Dataset Information

0

Alpha-synuclein fragments trigger distinct aggregation pathways.


ABSTRACT: Aggregation of alpha-synuclein (?Syn) is a crucial event underlying the pathophysiology of synucleinopathies. The existence of various intracellular and extracellular ?Syn species, including cleaved ?Syn, complicates the quest for an appropriate therapeutic target. Hence, to develop efficient disease-modifying strategies, it is fundamental to achieve a deeper understanding of the relevant spreading and toxic ?Syn species. Here, we describe comparative and proof-of-principle approaches to determine the involvement of ?Syn fragments in intercellular spreading. We demonstrate that two different ?Syn fragments (1-95 and 61-140) fulfill the criteria of spreading species. They efficiently instigate formation of proteinase-K-resistant aggregates from cell-endogenous full-length ?Syn, and drive it into different aggregation pathways. The resulting aggregates induce cellular toxicity. Strikingly, these aggregates are only detectable by specific antibodies. Our results suggest that ?Syn fragments might be relevant not only for spreading, but also for aggregation-fate determination and differential strain formation.

SUBMITTER: Chakroun T 

PROVIDER: S-EPMC6997403 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications


Aggregation of alpha-synuclein (αSyn) is a crucial event underlying the pathophysiology of synucleinopathies. The existence of various intracellular and extracellular αSyn species, including cleaved αSyn, complicates the quest for an appropriate therapeutic target. Hence, to develop efficient disease-modifying strategies, it is fundamental to achieve a deeper understanding of the relevant spreading and toxic αSyn species. Here, we describe comparative and proof-of-principle approaches to determi  ...[more]

Similar Datasets

| S-EPMC7037551 | biostudies-literature
| S-EPMC5019199 | biostudies-literature
| S-EPMC6117395 | biostudies-literature
| S-EPMC8558257 | biostudies-literature
| S-EPMC7460339 | biostudies-literature
| S-EPMC11334933 | biostudies-literature
| S-EPMC4317028 | biostudies-literature
| S-EPMC8392020 | biostudies-literature
| S-EPMC10216457 | biostudies-literature
| S-EPMC6842937 | biostudies-literature