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Large-scale mapping of branchpoints in human pre-mRNA transcripts in vivo.

ABSTRACT: We present the first large-scale identification of lariats-the transient branched introns that are released as a byproduct of pre-mRNA splicing. The locations of the branchpoints in these introns provide insight into the early steps of splicing. From this data set, we have developed a comprehensive model of 3' splice-site selection, identified new mechanisms of alternative splicing and mapped the distribution of splicing factors around branchpoints.

SUBMITTER: Taggart AJ

PROVIDER: S-EPMC3465671 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature

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Large-scale mapping of branchpoints in human pre-mRNA transcripts in vivo.

Taggart Allison J AJ DeSimone Alec M AM Shih Janice S JS Filloux Madeleine E ME Fairbrother William G WG

Nature structural & molecular biology 20120617 7

We present the first large-scale identification of lariats-the transient branched introns that are released as a byproduct of pre-mRNA splicing. The locations of the branchpoints in these introns provide insight into the early steps of splicing. From this data set, we have developed a comprehensive model of 3' splice-site selection, identified new mechanisms of alternative splicing and mapped the distribution of splicing factors around branchpoints. ...[more]

PMID: 22705790

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