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Diverse transcriptional initiation revealed by fine, large-scale mapping of mRNA start sites.

ABSTRACT: Determination of the mRNA start site is the first step in identifying the promoter region, which is of key importance for transcriptional regulation of gene expression. The 'oligo-capping' method enabled us to introduce a sequence tag to the first base of an mRNA by replacing the cap structure of the mRNA. Using cDNA libraries made from oligo-capped mRNAs, we could identify the transcriptional start site of an individual mRNA just by sequencing the 5'-end of the cDNA. The fine mapping of transcriptional start sites was performed for 5880 mRNAs in 276 human genes. Contrary to our expectations, the majority of the genes showed a diverse distribution of transcriptional start sites. They were distributed over 61.7 bp with a standard deviation of 19.5. Our finding may reflect the dynamic nature of transcriptional initiation events of human genes in vivo.

SUBMITTER: Suzuki Y

PROVIDER: S-EPMC1083880 | biostudies-other | 2001 May

REPOSITORIES: biostudies-other

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Diverse transcriptional initiation revealed by fine, large-scale mapping of mRNA start sites.

Suzuki Y Y Taira H H Tsunoda T T Mizushima-Sugano J J Sese J J Hata H H Ota T T Isogai T T Tanaka T T Morishita S S Okubo K K Sakaki Y Y Nakamura Y Y Suyama A A Sugano S S

EMBO reports 20010501 5

Determination of the mRNA start site is the first step in identifying the promoter region, which is of key importance for transcriptional regulation of gene expression. The 'oligo-capping' method enabled us to introduce a sequence tag to the first base of an mRNA by replacing the cap structure of the mRNA. Using cDNA libraries made from oligo-capped mRNAs, we could identify the transcriptional start site of an individual mRNA just by sequencing the 5'-end of the cDNA. The fine mapping of transcr ...[more]

PMID: 11375929

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