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Creating an infrastructure for high-throughput high-resolution cryogenic electron microscopy.

ABSTRACT: New instrumentation for three-dimensional electron microscopy is facilitating an increase in the throughput of data collection and reconstruction. The increase in throughput creates bottlenecks in the workflow for storing and processing the image data. Here we describe the creation and quantify the throughput of a high-throughput infrastructure supporting collection of three-dimensional data collection.

SUBMITTER: Shrum DC

PROVIDER: S-EPMC3466351 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature

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Creating an infrastructure for high-throughput high-resolution cryogenic electron microscopy.

Shrum Donald C DC Woodruff Brent W BW Stagg Scott M SM

Journal of structural biology 20120725 1

New instrumentation for three-dimensional electron microscopy is facilitating an increase in the throughput of data collection and reconstruction. The increase in throughput creates bottlenecks in the workflow for storing and processing the image data. Here we describe the creation and quantify the throughput of a high-throughput infrastructure supporting collection of three-dimensional data collection. ...[more]

PMID: 22842049

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