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Analytical High-resolution Electron Microscopy Reveals Organ-specific Nanoceria Bioprocessing.

ABSTRACT: This is the first utilization of advanced analytical electron microscopy methods, including high-resolution transmission electron microscopy, high-angle annular dark field scanning transmission electron microscopy, electron energy loss spectroscopy, and energy-dispersive X-ray spectroscopy mapping to characterize the organ-specific bioprocessing of a relatively inert nanomaterial (nanoceria). Liver and spleen samples from rats given a single intravenous infusion of nanoceria were obtained after prolonged (90 days) in vivo exposure. These advanced analytical electron microscopy methods were applied to elucidate the organ-specific cellular and subcellular fate of nanoceria after its uptake. Nanoceria is bioprocessed differently in the spleen than in the liver.

SUBMITTER: Graham UM

PROVIDER: S-EPMC5954437 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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Analytical High-resolution Electron Microscopy Reveals Organ-specific Nanoceria Bioprocessing.

Graham Uschi M UM Yokel Robert A RA Dozier Alan K AK Drummy Lawrence L Mahalingam Krishnamurthy K Tseng Michael T MT Birch Eileen E Fernback Joseph J

Toxicologic pathology 20171116 1

This is the first utilization of advanced analytical electron microscopy methods, including high-resolution transmission electron microscopy, high-angle annular dark field scanning transmission electron microscopy, electron energy loss spectroscopy, and energy-dispersive X-ray spectroscopy mapping to characterize the organ-specific bioprocessing of a relatively inert nanomaterial (nanoceria). Liver and spleen samples from rats given a single intravenous infusion of nanoceria were obtained after ...[more]

PMID: 29145781

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