Spi-1/PU.1 activates transcription through clustered DNA occupancy in erythroleukemia.

ABSTRACT: Acute leukemias are characterized by deregulation of transcriptional networks that control the lineage specificity of gene expression. The aberrant overexpression of the Spi-1/PU.1 transcription factor leads to erythroleukemia. To determine how Spi-1 mechanistically influences the transcriptional program, we combined a ChIP-seq analysis with transcriptional profiling in cells from an erythroleukemic mouse model. We show that Spi-1 displays a selective DNA-binding that does not often cause transcriptional modulation. We report that Spi-1 controls transcriptional activation and repression partially through distinct Spi-1 recruitment to chromatin. We revealed several parameters impacting on Spi-1-mediated transcriptional activation. Gene activation is facilitated by Spi-1 occupancy close to t

SUBMITTER: Ridinger-Saison M 

PROVIDER: S-EPMC3467057 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature