Ontology highlight
ABSTRACT:
SUBMITTER: Keyvanfar K
PROVIDER: S-EPMC3471523 | biostudies-literature | 2012 Oct
REPOSITORIES: biostudies-literature
Keyvanfar Keyvan K Weed Jason J Swamy Prashanth P Kajigaya Sachiko S Calado Rodrigo T RT Young Neal S NS
Blood 20120829 15
A 2-day method using flow cytometry and FISH for interphase cells was developed to detect monosomy 7 cells in myelodysplastic syndrome patients. The method, Interphase Chromosome Flow-FISH (IC Flow-FISH), involves fixation of leukocytes from blood, membrane permeabilization, hybridization of cellular DNA with peptide nucleic acid probes with cells intact, and analysis by flow cytometry. Hundreds to thousands of monosomy 7 cells were consistently detected from 10-20 mL of blood in patients with m ...[more]