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Synthetic polymer coatings for long-term growth of human embryonic stem cells.

ABSTRACT: We report a fully defined synthetic polymer coating, poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH), which sustains long-term human embryonic stem (hES) cell growth in several different culture media, including commercially available defined media. The development of a standardized, controllable and sustainable culture matrix for hES cells is an essential step in elucidating mechanisms that control hES cell behavior and in optimizing conditions for biomedical applications of hES cells.

SUBMITTER: Villa-Diaz LG 

PROVIDER: S-EPMC3471651 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature

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Synthetic polymer coatings for long-term growth of human embryonic stem cells.

Villa-Diaz Luis G LG   Nandivada Himabindu H   Ding Jun J   Nogueira-de-Souza Naiara C NC   Krebsbach Paul H PH   O'Shea K Sue KS   Lahann Joerg J   Smith Gary D GD  

Nature biotechnology 20100530 6

We report a fully defined synthetic polymer coating, poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH), which sustains long-term human embryonic stem (hES) cell growth in several different culture media, including commercially available defined media. The development of a standardized, controllable and sustainable culture matrix for hES cells is an essential step in elucidating mechanisms that control hES cell behavior and in optimizing conditions for biomedical  ...[more]

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