Dataset Information
Differential expression of phospholipase C epsilon 1 is associated with chronic atrophic gastritis and gastric cancer.
Ontology highlight
ABSTRACT: Background
Chronic inflammation plays a causal role in gastric tumor initiation. The identification of predictive biomarkers from gastric inflammation to tumorigenesis will help us to distinguish gastric cancer from atrophic gastritis and establish the diagnosis of early-stage gastric cancer. Phospholipase C epsilon 1 (PLC?1) is reported to play a vital role in inflammation and tumorigenesis. This study was aimed to investigate the clinical significance of PLC?1 in the initiation and progression of gastric cancer.Methodology/principal findings
Firstly, the mRNA and protein expression of PLC?1 were analyzed by reverse transcription-PCR and Western blotting in normal gastric mucous epithelial cell line GES-1 and gastric cancer cell lines AGS, SGC7901, and MGC803. The results showed both mRNA and protein levels of PLC?1 were up-regulated in gastric cancer cells compared with normal gastric mucous epithelial cells. Secondly, this result was confirmed by immunohistochemical detection in a tissue microarray including 74 paired gastric cancer and adjacent normal tissues. Thirdly, an independence immunohistochemical analysis of 799 chronic atrophic gastritis tissue specimens demonstrated that PLC?1 expression in atrophic gastritis tissues were down-regulated since PLC?1 expression was negative in 524 (65.6%) atrophic gastritis. In addition, matched clinical tissues from atrophic severe gastritis and gastric cancer patients were used to further confirm the previous results by analyzing mRNA and protein levels expression of PLC?1 in clinical samples.Conclusions/significances
Our results suggested that PLC?1 protein may be a potential biomarker to distinguish gastric cancer from inflammation lesion, and could have great potential in applications such as diagnosis and pre-warning of early-stage gastric cancer.
SUBMITTER: Chen J
PROVIDER: S-EPMC3471869 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Publications
Differential expression of phospholipase C epsilon 1 is associated with chronic atrophic gastritis and gastric cancer.
PloS one 20121015 10
<h4>Background</h4>Chronic inflammation plays a causal role in gastric tumor initiation. The identification of predictive biomarkers from gastric inflammation to tumorigenesis will help us to distinguish gastric cancer from atrophic gastritis and establish the diagnosis of early-stage gastric cancer. Phospholipase C epsilon 1 (PLCε1) is reported to play a vital role in inflammation and tumorigenesis. This study was aimed to investigate the clinical significance of PLCε1 in the initiation and pro ...[more]
