Ontology highlight
ABSTRACT:
SUBMITTER: Creek DJ
PROVIDER: S-EPMC3472505 | biostudies-literature | 2012 Oct
REPOSITORIES: biostudies-literature
Analytical chemistry 20120925 20
The combination of high-resolution LC-MS-based untargeted metabolomics with stable isotope tracing provides a global overview of the cellular fate of precursor metabolites. This methodology enables detection of putative metabolites from biological samples and simultaneous quantification of the pattern and extent of isotope labeling. Labeling of Trypanosoma brucei cell cultures with 50% uniformly (13)C-labeled glucose demonstrated incorporation of glucose-derived carbon into 187 of 588 putatively ...[more]