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Identification of rat Rosa26 locus enables generation of knock-in rat lines ubiquitously expressing tdTomato.


ABSTRACT: Recent discovery of a method for derivation and culture of germline-competent rat pluripotent stem cells (PSCs) enables generation of transgenic rats or knock-out rats via genetic modification of such PSCs. This opens the way to use rats, as is routine in mice, for analyses of gene functions or physiological features. In mouse or human, one widely used technique to express a gene of interest stably and ubiquitously is to insert that gene into the Rosa26 locus via gene targeting of PSCs. Rosa26 knock-in mice conditionally expressing a reporter or a toxin gene have contributed to tracing or ablation of specific cell lineages. We successfully identified a rat orthologue of the mouse Rosa26 locus. Insertion of tdTomato, a variant of red fluorescent protein, into the Rosa26 locus of PSCs of various rat strains allows ubiquitous expression of tdTomato. Through germline transmission of one Rosa26-tdTomato knock-in embryonic stem cell line, we also obtained tdTomato knock-in rats. These expressed tdTomato ubiquitously throughout their bodies, which indicates that the rat Rosa26 locus conserves functions of its orthologues in mouse and human. The new tools described here (targeting vectors, knock-in PSCs, and rats) should be useful for a variety of research using rats.

SUBMITTER: Kobayashi T 

PROVIDER: S-EPMC3475147 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature

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Identification of rat Rosa26 locus enables generation of knock-in rat lines ubiquitously expressing tdTomato.

Kobayashi Toshihiro T   Kato-Itoh Megumi M   Yamaguchi Tomoyuki T   Tamura Chihiro C   Sanbo Makoto M   Hirabayashi Masumi M   Nakauchi Hiromitsu H  

Stem cells and development 20120611 16


Recent discovery of a method for derivation and culture of germline-competent rat pluripotent stem cells (PSCs) enables generation of transgenic rats or knock-out rats via genetic modification of such PSCs. This opens the way to use rats, as is routine in mice, for analyses of gene functions or physiological features. In mouse or human, one widely used technique to express a gene of interest stably and ubiquitously is to insert that gene into the Rosa26 locus via gene targeting of PSCs. Rosa26 k  ...[more]

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