Ontology highlight
ABSTRACT:
SUBMITTER: Imayoshi I
PROVIDER: S-EPMC7035371 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Imayoshi Itaru I Tabuchi Sawako S Matsumoto Mami M Kitano Satsuki S Miyachi Hitoshi H Kageyama Ryoichiro R Yamanaka Akihiro A
Scientific reports 20200221 1
An engineered light-inducible chloride pump, Natronomonas pharaonis halorhodopsin 3 (eNpHR3) enables temporally and spatially precise inhibition of genetically defined cell populations in the intact nervous tissues. In this report, we show the generation of new mouse strains that express eNpHR3-EYFP fusion proteins after Cre- and/or Flp-mediated recombination to optically inhibit neuronal activity. In these mouse strains, Cre/Flp recombination induced high levels of opsin expression. We confirme ...[more]