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Faster STORM using compressed sensing.

ABSTRACT: In super-resolution microscopy methods based on single-molecule switching, the rate of accumulating single-molecule activation events often limits the time resolution. Here we developed a sparse-signal recovery technique using compressed sensing to analyze images with highly overlapping fluorescent spots. This method allows an activated fluorophore density an order of magnitude higher than what conventional single-molecule fitting methods can handle. Using this method, we demonstrated imaging microtubule dynamics in living cells with a time resolution of 3 s.

SUBMITTER: Zhu L 

PROVIDER: S-EPMC3477591 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature

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Faster STORM using compressed sensing.

Zhu Lei L   Zhang Wei W   Elnatan Daniel D   Huang Bo B  

Nature methods 20120422 7

In super-resolution microscopy methods based on single-molecule switching, the rate of accumulating single-molecule activation events often limits the time resolution. Here we developed a sparse-signal recovery technique using compressed sensing to analyze images with highly overlapping fluorescent spots. This method allows an activated fluorophore density an order of magnitude higher than what conventional single-molecule fitting methods can handle. Using this method, we demonstrated imaging mi  ...[more]