Faster and less phototoxic 3D fluorescence microscopy using a versatile compressed sensing scheme.

ABSTRACT: Three-dimensional fluorescence microscopy based on Nyquist sampling of focal planes faces harsh trade-offs between acquisition time, light exposure, and signal-to-noise. We propose a 3D compressed sensing approach that uses temporal modulation of the excitation intensity during axial stage sweeping and can be adapted to fluorescence microscopes without hardware modification. We describe implementations on a lattice light sheet microscope and an epifluorescence microscope, and show that images of beads and biological samples can be reconstructed with a 5-10 fold reduction of light exposure and acquisition time. Our scheme opens a new door towards faster and less damaging 3D fluorescence microscopy.

SUBMITTER: Woringer M 

PROVIDER: S-EPMC5499637 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature