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VDAC3 regulates centriole assembly by targeting Mps1 to centrosomes.

ABSTRACT: Centrioles are duplicated during S-phase to generate the two centrosomes that serve as mitotic spindle poles during mitosis. The centrosomal pool of the Mps1 kinase is important for centriole assembly, but how Mps1 is delivered to centrosomes is unknown. Here we have identified a centrosome localization domain within Mps1 and identified the mitochondrial porin VDAC3 as a protein that binds to this region of Mps1. Moreover, we show that VDAC3 is present at the mother centriole and modulates centriole assembly by recruiting Mps1 to centrosomes.

SUBMITTER: Majumder S

PROVIDER: S-EPMC3478317 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature

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VDAC3 regulates centriole assembly by targeting Mps1 to centrosomes.

Majumder Shubhra S Slabodnick Mark M Pike Amanda A Marquardt Joseph J Fisk Harold A HA

Cell cycle (Georgetown, Tex.) 20120830 19

Centrioles are duplicated during S-phase to generate the two centrosomes that serve as mitotic spindle poles during mitosis. The centrosomal pool of the Mps1 kinase is important for centriole assembly, but how Mps1 is delivered to centrosomes is unknown. Here we have identified a centrosome localization domain within Mps1 and identified the mitochondrial porin VDAC3 as a protein that binds to this region of Mps1. Moreover, we show that VDAC3 is present at the mother centriole and modulates centr ...[more]

PMID: 22935710

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