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Analysis of in vitro secretion profiles from adipose-derived cell populations.


ABSTRACT: BACKGROUND: Adipose tissue is an attractive source of cells for therapeutic purposes because of the ease of harvest and the high frequency of mesenchymal stem cells (MSCs). Whilst it is clear that MSCs have significant therapeutic potential via their ability to secrete immuno-modulatory and trophic cytokines, the therapeutic use of mixed cell populations from the adipose stromal vascular fraction (SVF) is becoming increasingly common. METHODS: In this study we have measured a panel of 27 cytokines and growth factors secreted by various combinations of human adipose-derived cell populations. These were 1. co-culture of freshly isolated SVF with adipocytes, 2. freshly isolated SVF cultured alone, 3. freshly isolated adipocytes alone and 4. adherent adipose-derived mesenchymal stem cells (ADSCs) at passage 2. In addition, we produced an 'in silico' dataset by combining the individual secretion profiles obtained from culturing the SVF with that of the adipocytes. This was compared to the secretion profile of co-cultured SVF and adipocytes. Two-tailed t-tests were performed on the secretion profiles obtained from the SVF, adipocytes, ADSCs and the 'in silico' dataset and compared to the secretion profiles obtained from the co-culture of the SVF with adipocytes. A p-value of?

SUBMITTER: Blaber SP 

PROVIDER: S-EPMC3479070 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Analysis of in vitro secretion profiles from adipose-derived cell populations.

Blaber Sinead P SP   Webster Rebecca A RA   Hill Cameron J CJ   Breen Edmond J EJ   Kuah Donald D   Vesey Graham G   Herbert Benjamin R BR  

Journal of translational medicine 20120822


<h4>Background</h4>Adipose tissue is an attractive source of cells for therapeutic purposes because of the ease of harvest and the high frequency of mesenchymal stem cells (MSCs). Whilst it is clear that MSCs have significant therapeutic potential via their ability to secrete immuno-modulatory and trophic cytokines, the therapeutic use of mixed cell populations from the adipose stromal vascular fraction (SVF) is becoming increasingly common.<h4>Methods</h4>In this study we have measured a panel  ...[more]

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