Ontology highlight
ABSTRACT:
SUBMITTER: Untergasser A
PROVIDER: S-EPMC3480454 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Untergasser Andreas A Bijl Gerben J M GJ Liu Wei W Bisseling Ton T Schaart Jan G JG Geurts René R
PloS one 20121024 10
Advancement in plant research is becoming impaired by the fact that the transfer of multiple genes is difficult to achieve. Here we present a new binary vector for Agrobacterium tumefaciens mediated transformation, pHUGE-Red, in concert with a cloning strategy suited for the transfer of up to nine genes at once. This vector enables modular cloning of large DNA fragments by employing Gateway technology and contains DsRED1 as visual selection marker. Furthermore, an R/Rs inducible recombination sy ...[more]