Ontology highlight
ABSTRACT:
SUBMITTER: Burns KE
PROVIDER: S-EPMC3481346 | biostudies-literature | 2012 Oct
REPOSITORIES: biostudies-literature
Burns Kristin E KE McAllister Fiona E FE Schwerdtfeger Carsten C Mintseris Julian J Cerda-Maira Francisca F Noens Elke E EE Wilmanns Matthias M Hubbard Stevan R SR Melandri Francesco F Ovaa Huib H Gygi Steven P SP Darwin K Heran KH
The Journal of biological chemistry 20120831 44
Deamidase of Pup (Dop), the prokaryotic ubiquitin-like protein (Pup)-deconjugating enzyme, is critical for the full virulence of Mycobacterium tuberculosis and is unique to bacteria, providing an ideal target for the development of selective chemotherapies. We used a combination of genetics and chemical biology to characterize the mechanism of depupylation. We identified an aspartate as a potential nucleophile in the active site of Dop, suggesting a novel protease activity to target for inhibito ...[more]