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Rapid Detection of Escherichia coli O157: H7 by Fluorescent Amplification-Based Specific Hybridization (FLASH) PCR.

ABSTRACT: BACKGROUND:Escherichia coli O157:H7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans. METHODS:We developed fluorescent amplification-based specific hybridization (FLASH-PCR) assay to detect the Stx-encoding gene Stx-1 of E. coli O157:H7. RESULT:PCR product of 336 bp was successfully amplified in a FLASH-PCR. CONCLUSION:As rapid detection and confirmation of the presence of E. coli O157:H7 are of importance for the medical, food, and water industries, FLASH-PCR is one of selective methods for detection of E. coli O157:H7.

SUBMITTER: Khatami F

PROVIDER: S-EPMC3482334 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature

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Rapid Detection of Escherichia coli O157: H7 by Fluorescent Amplification-Based Specific Hybridization (FLASH) PCR.

Khatami F F Heidari M M Khatami M M

Iranian Red Crescent medical journal 20120930 9

<h4>Background</h4>Escherichia coli O157:H7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans.<h4>Methods</h4>We developed fluorescent amplification-based specific hybridization (FLASH-PCR) assay to detect the Stx-encoding gene Stx-1 of E. coli O157:H7.<h4>Result</h4>PCR product of 336 bp was successfully amplified in a FLASH-PCR.<h4>Conclusion</h4>As rapid detection and conf ...[more]

PMID: 23115724

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