Ontology highlight
ABSTRACT:
SUBMITTER: Goodridge L
PROVIDER: S-EPMC91198 | biostudies-literature | 1999 Apr
REPOSITORIES: biostudies-literature
Goodridge L L Chen J J Griffiths M M
Applied and environmental microbiology 19990401 4
In this paper we describe evaluation and characterization of a novel assay that combines immunomagnetic separation and a fluorescently stained bacteriophage for detection of Escherichia coli O157:H7 in broth. When it was combined with flow cytometry, the fluorescent-bacteriophage assay (FBA) was capable of detecting 10(4) cells/ml. A modified direct epifluorescent-filter technique (DEFT) was employed in an attempt to estimate bacterial concentrations. Using regression analysis, we calculated tha ...[more]