Ontology highlight
ABSTRACT:
SUBMITTER: Wood AJ
PROVIDER: S-EPMC3489282 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Wood Andrew J AJ Lo Te-Wen TW Zeitler Bryan B Pickle Catherine S CS Ralston Edward J EJ Lee Andrew H AH Amora Rainier R Miller Jeffrey C JC Leung Elo E Meng Xiangdong X Zhang Lei L Rebar Edward J EJ Gregory Philip D PD Urnov Fyodor D FD Meyer Barbara J BJ
Science (New York, N.Y.) 20110623 6040
Evolutionary studies necessary to dissect diverse biological processes have been limited by the lack of reverse genetic approaches in most organisms with sequenced genomes. We established a broadly applicable strategy using zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) for targeted disruption of endogenous genes and cis-acting regulatory elements in diverged nematode species. ...[more]