Spontaneous structural changes in actin regulate G-F transformation.
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ABSTRACT:
SUBMITTER: Morimatsu M
PROVIDER: S-EPMC3489878 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Publications
Spontaneous structural changes in actin regulate G-F transformation.
Morimatsu Masatoshi M Togashi Yuichi Y Nishikawa So S Sugawa Mitsuhiro M Iwane Atsuko H AH Yanagida Toshio T
PloS one 20121105 11
Transformations between G- (monomeric) and F-actin (polymeric) are important in cellular behaviors such as migration, cytokinesis, and morphing. In order to understand these transitions, we combined single-molecule Förster resonance energy transfer with total internal reflection fluorescence microscopy to examine conformational changes of individual actin protomers. We found that the protomers can take different conformational states and that the transition interval is in the range of hundreds o ...[more]
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