Dataset Information
Intraoperative fluorescence imaging of peripheral and central nerves through a myelin-selective contrast agent.
Ontology highlight
ABSTRACT: Purpose
Patients suffer from complications as a result of unintentional nerve damage during surgery. We focus on improving intraoperative visualization of nerves through the use of a targeted fluorophore and optical imaging instrumentation.Procedure
A myelin-targeting fluorophore, GE3111, was synthesized, characterized for its optical and myelin-binding properties using purified myelin basic protein, and evaluated in mice. Additionally, a compact instrument was adapted to visualize nerves.Results
GE3111 was synthesized using a versatile methodology. Its optical properties were sensitive to the local environment both in vitro and in vivo. Following intravenous injection, central and peripheral nerves were visualized, with the kinetics of nerve uptake modifiable depending on the formulation. Fluorescence polarization showed specific and strong binding to purified myelin basic protein. Nerves were visualized in vivo using a dedicated compact imaging device requiring less than 2.5 mW/cm(2) of illumination at 405 nm.Conclusions
Fluorescence imaging of nerves through myelin showed a potential for use in image-guided surgery. Intraoperative nerve imaging is an example where contrast agent and instrument development come together as a result of clinical need.
SUBMITTER: Cotero VE
PROVIDER: S-EPMC3492698 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
Publications
Intraoperative fluorescence imaging of peripheral and central nerves through a myelin-selective contrast agent.
Molecular imaging and biology 20121201 6
<h4>Purpose</h4>Patients suffer from complications as a result of unintentional nerve damage during surgery. We focus on improving intraoperative visualization of nerves through the use of a targeted fluorophore and optical imaging instrumentation.<h4>Procedure</h4>A myelin-targeting fluorophore, GE3111, was synthesized, characterized for its optical and myelin-binding properties using purified myelin basic protein, and evaluated in mice. Additionally, a compact instrument was adapted to visuali ...[more]