Ontology highlight
ABSTRACT:
SUBMITTER: Raaijmakers JA
PROVIDER: S-EPMC3492733 | biostudies-literature | 2012 Nov
REPOSITORIES: biostudies-literature
Raaijmakers Jonne A JA van Heesbeen Roy G H P RG Meaders Johnathan L JL Geers Erica F EF Fernandez-Garcia Belen B Medema René H RH Tanenbaum Marvin E ME
The EMBO journal 20121002 21
The microtubule motor protein kinesin-5 (Eg5) provides an outward force on centrosomes, which drives bipolar spindle assembly. Acute inhibition of Eg5 blocks centrosome separation and causes mitotic arrest in human cells, making Eg5 an attractive target for anti-cancer therapy. Using in vitro directed evolution, we show that human cells treated with Eg5 inhibitors can rapidly acquire the ability to divide in the complete absence of Eg5 activity. We have used these Eg5-independent cells to study ...[more]