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Cytokine responses to novel antigens in an Indian population living in an area endemic for visceral leishmaniasis.


ABSTRACT:

Background

There are no effective vaccines for visceral leishmaniasis (VL), a neglected parasitic disease second only to malaria in global mortality. We previously identified 14 protective candidates in a screen of 100 Leishmania antigens as DNA vaccines in mice. Here we employ whole blood assays to evaluate human cytokine responses to 11 of these antigens, in comparison to known defined and crude antigen preparations.

Methods

Whole blood assays were employed to measure IFN-?, TNF-? and IL-10 responses to peptide pools of the novel antigens R71, Q51, L37, N52, L302.06, J89, M18, J41, M22, M63, M57, as well as to recombinant proteins of tryparedoxin peroxidase (TRYP), Leishmania homolog of the receptor for activated C kinase (LACK) and to crude soluble Leishmania antigen (SLA), in Indian patients with active (n = 8) or cured (n = 16) VL, and in modified Quantiferon positive (EHC(+ve), n = 20) or modified Quantiferon negative (EHC(-ve), n = 9) endemic healthy controls (EHC).

Results

Active VL, cured VL and EHC(+ve) groups showed elevated SLA-specific IFN-?, but only active VL patients produced IL-10 and EHC(+ve) did not make TNF-?. IFN-? to IL-10 and TNF-? to IL-10 ratios in response to TRYP and LACK antigens were higher in cured VL and EHC(+ve) exposed individuals compared to active VL. Five of the eleven novel candidates (R71, L37, N52, J41, and M22) elicited IFN-? and TNF-?, but not IL-10, responses in cured VL (55-87.5% responders) and EHC(+ve) (40-65% responders) subjects.

Conclusions

Our results are consistent with an important balance between pro-inflammatory IFN? and TNF? cytokine responses and anti-inflammatory IL-10 in determining outcome of VL in India, as highlighted by response to both crude and defined protein antigens. Importantly, cured VL patients and endemic Quantiferon positive individuals recognise 5 novel vaccine candidate antigens, confirming our recent data for L. chagasi in Brazil, and their potential as cross-species vaccine candidates.

SUBMITTER: Singh OP 

PROVIDER: S-EPMC3493615 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Publications

Cytokine responses to novel antigens in an Indian population living in an area endemic for visceral leishmaniasis.

Singh Om Prakash OP   Stober Carmel B CB   Singh Abhishek Kr AK   Blackwell Jenefer M JM   Sundar Shyam S  

PLoS neglected tropical diseases 20121025 10


<h4>Background</h4>There are no effective vaccines for visceral leishmaniasis (VL), a neglected parasitic disease second only to malaria in global mortality. We previously identified 14 protective candidates in a screen of 100 Leishmania antigens as DNA vaccines in mice. Here we employ whole blood assays to evaluate human cytokine responses to 11 of these antigens, in comparison to known defined and crude antigen preparations.<h4>Methods</h4>Whole blood assays were employed to measure IFN-γ, TNF  ...[more]

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