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Paxillin contracts the osteoclast cytoskeleton.


ABSTRACT: Osteoclastic bone resorption depends upon the cell's ability to organize its cytoskeleton via the ?v?3 integrin and osteoclastogenic cytokines. Because paxillin associates with ?v?3, we asked if it participates in skeletal degradation. Unlike deletion of other ?v?3-associated cytoskeleton-regulating molecules, which impairs the cell's ability to spread, paxillin-deficient (Pax(-/-) ) osteoclasts, generated from embryonic stem cells, "superspread" in response to receptor activator of NF-?B ligand (RANKL) and form large, albeit dynamically atypical, actin bands. Despite their increased size, Pax(-/-) osteoclasts resorb bone poorly, excavating pits approximately one-third normal depth. Ligand-occupied ?v?3 or RANKL promotes paxillin serine and tyrosine phosphorylation, the latter via cellular sarcoma (c-Src). The abnormal Pax(-/-) phenotype is rescued by wild-type (WT) paxillin but not that lacking its LD4 domain. In keeping with the appearance of mutant osteoclasts, WT paxillin, overexpressed in WT cells, contracts the cytoskeleton. Most importantly, the abnormal phenotype of Pax(-/-) osteoclasts likely represents failed RANKL-mediated delivery of myosin IIA to the actin cytoskeleton via the paxillin LD4 domain but is independent of tyrosine phosphorylation. Thus, in response to RANKL, paxillin associates with myosin IIA to contract the osteoclast cytoskeleton, thereby promoting its bone-degrading capacity.

SUBMITTER: Zou W 

PROVIDER: S-EPMC3494816 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature

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Paxillin contracts the osteoclast cytoskeleton.

Zou Wei W   Deselm Carl J CJ   Broekelmann Thomas J TJ   Mecham Robert P RP   Vande Pol Scott S   Choi Kyunghee K   Teitelbaum Steven L SL  

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 20121201 12


Osteoclastic bone resorption depends upon the cell's ability to organize its cytoskeleton via the αvβ3 integrin and osteoclastogenic cytokines. Because paxillin associates with αvβ3, we asked if it participates in skeletal degradation. Unlike deletion of other αvβ3-associated cytoskeleton-regulating molecules, which impairs the cell's ability to spread, paxillin-deficient (Pax(-/-) ) osteoclasts, generated from embryonic stem cells, "superspread" in response to receptor activator of NF-κB ligand  ...[more]

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