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Selective labeling of polypeptides using protein farnesyltransferase via rapid oxime ligation.


ABSTRACT: An aldehyde-containing alternative substrate for protein farnesyltransferase was prepared and shown to be enzymatically incorporated into a peptide and a protein. The protein was subsequently immobilized onto aminooxy-functionalized agarose beads or labeled with a fluorophore. This method for protein modification provides an alternative to the commonly employed Cu(I)-catalyzed click reaction.

SUBMITTER: Rashidian M 

PROVIDER: S-EPMC3495172 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature

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Selective labeling of polypeptides using protein farnesyltransferase via rapid oxime ligation.

Rashidian Mohammad M   Dozier Jonathan K JK   Lenevich Stepan S   Distefano Mark D MD  

Chemical communications (Cambridge, England) 20101022 47


An aldehyde-containing alternative substrate for protein farnesyltransferase was prepared and shown to be enzymatically incorporated into a peptide and a protein. The protein was subsequently immobilized onto aminooxy-functionalized agarose beads or labeled with a fluorophore. This method for protein modification provides an alternative to the commonly employed Cu(I)-catalyzed click reaction. ...[more]

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