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Lipid-coated hydrogel shapes as components of electrical circuits and mechanical devices.

ABSTRACT: Recently, two-dimensional networks of aqueous droplets separated by lipid bilayers, with engineered protein pores as functional elements, were used to construct millimeter-sized devices such as a light sensor, a battery, and half- and full-wave rectifiers. Here, for the first time, we show that hydrogel shapes, coated with lipid monolayers, can be used as building blocks for such networks, yielding scalable electrical circuits and mechanical devices. Examples include a mechanical switch, a rotor driven by a magnetic field and painted circuits, analogous to printed circuit boards, made with centimeter-length agarose wires. Bottom-up fabrication with lipid-coated hydrogel shapes is therefore a useful step towards the synthetic biology of functional devices including minimal tissues.

SUBMITTER: Sapra KT

PROVIDER: S-EPMC3497031 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Lipid-coated hydrogel shapes as components of electrical circuits and mechanical devices.

Sapra K Tanuj KT Bayley Hagan H

Scientific reports 20121114

Recently, two-dimensional networks of aqueous droplets separated by lipid bilayers, with engineered protein pores as functional elements, were used to construct millimeter-sized devices such as a light sensor, a battery, and half- and full-wave rectifiers. Here, for the first time, we show that hydrogel shapes, coated with lipid monolayers, can be used as building blocks for such networks, yielding scalable electrical circuits and mechanical devices. Examples include a mechanical switch, a rotor ...[more]

PMID: 23152939

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Project description:The development of next-generation transmembrane protein-based biosensors relies heavily on the use of black lipid membranes (BLMs); however, electrical, mechanical, and temporal instability of BLMs poses a limiting challenge to biosensor development. In this work, micrometer-sized glass apertures were modified with silanes of different chain length and fluorine composition, including 3-cyanopropyldimethychlorosilane (CPDCS), ethyldimethylchlorosilane (EDCS), n-octyldimethylchlorosilane (ODCS), (tridecafluoro-1, 1, 2, 2-tetrahydrooctyl)dimethylchlorosilane (PFDCS), or (heptadecafluoro-1,1,2,2-tetrahydrodecyl)dimethylchlorosilane (PFDDCS), to explore the effect of substrate surface energy on BLM stability. Low energy silane-modified surfaces promoted enhanced lipid-substrate interactions that facilitate the formation of low-leakage, stable BLMs. The surface energies of silane-modified substrates were 30 ± 3, 16 ± 1, 14 ± 2, 11 ± 1, and 7.1 ± 2 mJ m(-2) for CDCS, EDCS, ODCS, PFDCS, and PFDDCS, respectively. Decreased surface energy directly correlated to improved electrical, mechanical, and temporal BLM stability. Amphiphobic perfluorinated surface modifiers yielded superior performance compared to traditional hydrocarbon modifiers in terms of stability and BLM formation, with only marginal effects on BLM membrane permeability. Leakage currents obtained for PFDCS and PFDDCS BLMs were elevated only 10-30%, though PFDDCS modification yielded >5-fold increase in electrical stability as indicated by breakdown voltage (> 2000 mV vs 418 ± 73 mV), and >25-fold increase in mechanical stability as indicated by air-water transfers (> 50 vs 2 ± 0.2) when compared to previously reported CPDCS modification. Importantly, the dramatically improved membrane stabilities were achieved with no deleterious effects on reconstituted ion channel function, as evidenced by ?-hemolysin activity. Thus, this approach provides a simple, low cost, and broadly applicable alternative for BLM stabilization and should contribute significantly toward the development of next-generation ion-channel-functionalized biosensors.

Dataset Information

Lipid-coated hydrogel shapes as components of electrical circuits and mechanical devices.

Publications

Lipid-coated hydrogel shapes as components of electrical circuits and mechanical devices.

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