Dataset Information
Metabolism of insulin glargine after repeated daily subcutaneous injections in subjects with type 2 diabetes.
Ontology highlight
ABSTRACT: Objective
To investigate concentration of plasma insulin glargine after its subcutaneous dosing compared with concentration of its metabolites 1 (M1) and 2 (M2) in subjects with type 2 diabetes.Research design and methods
Nine subjects underwent a 32-h euglycemic glucose clamp study (0.4 units/kg glargine after 1 week of daily glargine administration). Glargine, M1, and M2 were measured by a specific liquid chromatography-tandem mass spectrometry assay.Results
Glargine was detected in only five of the nine subjects, at few time points, and at negligible concentrations. M1 was detected in all subjects and exhibited the same pattern as traditional radioimmunoassay-measured plasma insulin. M2 was not detected at all.Conclusions
After subcutaneous injection, glargine was minimally detectable in blood, whereas its metabolite M1 accounted for most (>90%) of the plasma insulin concentration and metabolic action of the injected glargine.
SUBMITTER: Lucidi P
PROVIDER: S-EPMC3507576 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
Publications
Metabolism of insulin glargine after repeated daily subcutaneous injections in subjects with type 2 diabetes.
Diabetes care 20121018 12
<h4>Objective</h4>To investigate concentration of plasma insulin glargine after its subcutaneous dosing compared with concentration of its metabolites 1 (M1) and 2 (M2) in subjects with type 2 diabetes.<h4>Research design and methods</h4>Nine subjects underwent a 32-h euglycemic glucose clamp study (0.4 units/kg glargine after 1 week of daily glargine administration). Glargine, M1, and M2 were measured by a specific liquid chromatography-tandem mass spectrometry assay.<h4>Results</h4>Glargine wa ...[more]