Novel conformation of an RNA structural switch.
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ABSTRACT:
SUBMITTER: Kennedy SD
PROVIDER: S-EPMC3508625 | biostudies-literature | 2012 Nov
REPOSITORIES: biostudies-literature
Publications
Novel conformation of an RNA structural switch.
Kennedy Scott D SD Kierzek Ryszard R Turner Douglas H DH
Biochemistry 20121112 46
The RNA duplex, (5'GACGAGUGUCA)(2), has two conformations in equilibrium. The nuclear magnetic resonance solution structure reveals that the major conformation of the loop, 5'GAGU/3'UGAG, is novel and contains two unusual Watson-Crick/Hoogsteen GG pairs with G residues in the syn conformation, two A residues stacked on each other in the center of the helix with inverted sugars, and two bulged-out U residues. The structure provides a benchmark for testing approaches for predicting local RNA struc ...[more]
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