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Expression, purification, crystallization and preliminary X-ray analysis of NAD(P)H-dependent carbonyl reductase specifically expressed in thyroidectomized chicken fatty liver.

ABSTRACT: An NAD(P)H-dependent carbonyl reductase specifically expressed in thyroidectomized chicken fatty liver was crystallized using the sitting-drop vapour-diffusion method with polyethylene glycol 300 as the precipitant. The crystals belonged to the monoclinic space group C2, with unit-cell parameters a=104.26, b=81.32, c=77.27?Å, ?=119.43°, and diffracted to 1.86?Å resolution on beamline NE3A at the Photon Factory. The overall Rmerge was 5.4% and the data completeness was 99.4%.

SUBMITTER: Yoneda K

PROVIDER: S-EPMC3509991 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature

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Expression, purification, crystallization and preliminary X-ray analysis of NAD(P)H-dependent carbonyl reductase specifically expressed in thyroidectomized chicken fatty liver.

Yoneda Kazunari K Fukuda Yudai Y Shibata Takeshi T Araki Tomohiro T Nikki Takahiro T Sakuraba Haruhiko H Ohshima Toshihisa T

Acta crystallographica. Section F, Structural biology and crystallization communications 20121128 Pt 12

An NAD(P)H-dependent carbonyl reductase specifically expressed in thyroidectomized chicken fatty liver was crystallized using the sitting-drop vapour-diffusion method with polyethylene glycol 300 as the precipitant. The crystals belonged to the monoclinic space group C2, with unit-cell parameters a=104.26, b=81.32, c=77.27 Å, β=119.43°, and diffracted to 1.86 Å resolution on beamline NE3A at the Photon Factory. The overall Rmerge was 5.4% and the data completeness was 99.4%. ...[more]

PMID: 23192050

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