Ontology highlight
ABSTRACT:
SUBMITTER: Marh J
PROVIDER: S-EPMC3511126 | biostudies-literature | 2012 Nov
REPOSITORIES: biostudies-literature
Marh Joel J Stoytcheva Zoia Z Urschitz Johann J Sugawara Atsushi A Yamashiro Hideaki H Owens Jesse B JB Stoytchev Ilko I Pelczar Pawel P Yanagimachi Ryuzo R Moisyadi Stefan S
Proceedings of the National Academy of Sciences of the United States of America 20121023 47
We have developed a unique method for mouse transgenesis. The transposase-enhanced pronuclear microinjection (PNI) technique described herein uses the hyperactive piggyBac transposase to insert a large transgene into the mouse genome. This procedure increased transgene integration efficiency by fivefold compared with conventional PNI or intracytoplasmic sperm injection-mediated transgenesis. Our data indicate that the transposase-enhanced PNI technique additionally requires fewer embryos to be m ...[more]