Sf-PHB2, a new transcription factor, drives WSSV Ie1 gene expression via a 12-bp DNA element.

ABSTRACT:

Background

The WSSV immediate early gene ie1 is highly expressed throughout viral infection cycle and may play a central role in initiating viral replication during infection.

Results

Here, a detailed characterization of the ie1 promoter was performed using deletion and mutation analyses to elucidate the role of the individual promoter motifs. Three results were obtained: 1) the ie1 promoter is a classical eukaryotic promoter that contains the initiator element (Inr) and TATA box responsible for the basal promoter activity; 2) mutation or truncation of a predicted Sp1 site decreased the level of promoter activity by about 3-fold, indicating that the Sp1 site is an important cis-element of the promoter; and 3) truncation of a 12-bp sequence that resides at -78/-67 of the ie1

SUBMITTER: Ma G 

PROVIDER: S-EPMC3511282 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature